ATTENTION ⚠️
On Saturday April 20th, 2024 at 8:00 AM (UTC), emergency site maintenance has been scheduled for iLab DFCI. Details and updates can be found on the Agilent Status Page. For additional information, please contact the Agilent iLab Support by clicking on 'Help' at the top of your account page or by emailing ilab-support@agilent.com.
Molecular Biology Core Facilities: Genomics Core Services
Illumina MiSeq Sequencing
~16M reads
Run Programs
MiSeq Pipeline
Consultation
If it is your first time running MiSeq samples at the MBCF or you have something new that you would like to try please Contact Us for a brief consultation.
DNA Library Considerations and Requirements
The library should consist of DNA fragments that are between 200-800bp with Illumina PE adapter sequences. For optimal clustering and basecalling it helps to have a relatively narrow size range (~200bp). Even if you are only running single-end sequencing, the MiSeq requires PE adapters for cluster generation. Libaries prepped for HiSeq or GAII single end runs may NOT be compatible with the MiSeq. Libraries with low base diversity are also problematic and can require phiX spike-in of 30%-60%, or an alternative library construction approach for successful clustering and sequencing. Email to ask about our Nextera Library Prep Service
DNA Library QC
MBCF will spec samples on Qubit fluormeter to determine concentration of dsDNA and analyze on a TapeStation for fragment size information to estimate library molarity and to determine appropriate loading amount for optimal cluster generation. qPCR will be available soon as well.
Sequencing
Actual run times vary from 4hrs to 40hrs depending on the number of cycles requested. Our turnaround time is usually within 2-3 days of receiving the sample and sometimes as fast as same day for shorter runs.
Data Transfer
Upon run completion fast will be made available on our webserver along with instructions for access. You may request an Illumina BaseSpace link to you. We can also provide data alignment to a reference sequence (this should be addressed during the consultation)
Nextera Illumina Library prep
MBCF Genomics Instrumentation
3 Illumina MiSeqs (Single Lane NGS)
Agilent TapeStation (DNA size analysis)
Sage Science Pippin Prep (DNA Size Selection)
Qubit Fluorometer
Sample Drop-off Locations
Samples can be dropped off in the MBCF Fish room near 3rd floor elevator lobby of Smith, directly to our lab on the 4th floor of DFCI Fenway Campus, or shipped via FedEx.
Physical Street Address / Fedex Delivery:
Molecular Biology Core Facilities
Dana Farber Cancer Institute North Campus
21-27 Burlington Avenue 4th Floor
Boston, MA 02215
USA
US postal, UPS, Mailing Address:
Molecular Biology Core Facilities
Dana Farber Cancer Institute North Campus
450 Brookline Avenue, BA404
Boston, MA 02215
USA
Purchase Orders are to be made out to:
Dana Farber Cancer Institute
General Accounting, BP-425
450 Brookline Avenue
Boston, MA 02215-5450
Note to Partners, Harvard, Boston Children’s and Tufts researchers:
If you are using a PO from an institution other than one under which you signed in, you must stop and contact the DFCI iLab staff at sc_billing@dfci.harvard.edu
Name | Role | Phone | Location | |
---|---|---|---|---|
Zach Herbert |
Associate Director Genomics Core Facility
|
617-632-3082
|
zherbert@mail.dfci.harvard.edu
|
|
Paul Morrison |
Director
|
617-632-3082
|
paul_morrison@dfci.harvard.edu
|
Initiate Service Requests |
► Illumina MiSeq Next Gen Sequencing (1) | |||
Name | Description | Price | |
---|---|---|---|
Illumina MiSeq 150 v3 Sequencing |
The MiSeq 150 v3 cycle kit contains 150 cycles of sequencing and can be broken up in many ways! Please be sure to indicate on the form what your read length requirements are. If you are unsure, don't hesitate to ask.
Data Output: 22-25M reads Sample Requirement: >10nM in >10ul (if this is problem contact us) MBCF QC: Qubit, TapeStation, qPCR Turnaround time: 3-7 days |
Inquire | |
► Libraries for NovaSeq sequencing (1) | |||
Name | Description | Price | |
Libraries for NovaSeq sequencing |
Some uniquely dual-indexed libraries are eligible for sequencing on our NovaSeq 6000 sequencer, PE150. Please inquire with MBCF team.
$250/100M reads (internal) $300/100M reads (external) |
Inquire | |
► Archive (1) | |||
Name | Description | Price | |
PCR Library Indexing (Nextera) | Inquire |
View Larger Map
Click on this map for specific directions to and from Logan Airport and the Mass Pike as well as a good place to eat while visiting MBCF.