Core - Hematologic Neoplasia Flow Cytometry

 

Welcome to the Division of Hematologic Neoplasia Flow Cytometry Core Facility at the Dana Farber Cancer Institute in Boston, MA.

The facility is located on the 5th floor of the Mayer building, in rooms 522a, 583 and 584.

Please visit the Hematologic Neoplasia Flow Core Website for more information.

 

Equipment

Sorters

 Analyzers

Rates

DFCI Laboratories                   $85.00/hour

External Laboratories             $120.00/hour

Corporate Rate                       $150.00/hour

Rates are calculated in 1.0 hour increments.

 

DFCI Laboratories                  $65.00/hour

External Laboratories             $85.00/hour

Corporate Rate                       $120.00/hour

Rates are calculated in 0.5 hour increments.

Instruments

BD FACSAria II

  • Mayer 584
  • 4 Lasers
  • 15 Parameters
  • 4 Way Sorting

 

BD FACSAria II UV

  • Mayer 584
  • 5 Lasers
  • 18 Parameters 
  • 4 Way Sorting

 

BD LSR Fortessa SORP

(Fortessa 1, Fortessa 2, and Fortessa X-20)

  • Mayer 522a
  • 4 Lasers
  • 17 Parameters
  • HTS Plate Reader

BD FACSCanto II Carousel

(Canto A)

  • Mayer 583
  • 3 Lasers
  • 8 Parameters
  • Carousel Tube Loader

BD FACSCanto II HTS

(Canto B and Canto C)

  • Mayer 583
  • 3 Lasers
  • 8 Parameters
  • HTS Plate Reader

The Hematologic Neoplasia Flow Core is a BL2+ Facility

Lentivirus and Human Primary Samples can be sorted. (Sorters are housed in Bioprotect hoods)

Lentivirus and Human Primary Samples can only be analyzed if they are fixed and in plates. 

The Flow Core will not sort any samples containing known pathogens. 

 

Users External to DFCI

Purchase Orders are to be made out to:

Dana Farber Cancer Institute

General Accounting, BP-425

450 Brookline Avenue

Boston, MA 02215-5450

 

Note to Partners, Harvard, Boston Children’s and Tufts researchers:

Partners Health Care (includes MGH, BWH, McLean, Spaulding, etc): choose vendor 0000008573

Harvard (includes HMS,Dental, Wyss, HSPH, etc.): choose vendor site: 01_PRIMARY

Tufts University: choose Supplier 0000000655

Boston Children’s Hospital: choose vendor 0000001886

 

Laboratory Contact

Laboratory Address:

Hematologic Neoplasia Flow Cytometry Core Facility

Attn: Suzan Lazo

Dana-Farber Cancer Institute

450 Brookline Ave.

Mayer 584

Boston, MA 02215

 

Laboratory Telephone & Email:

Email: hemneo_flow@dfci.harvard.edu

Phone: 617-632-4571

Fax: 617-632-5167


Our Website:  http://flowcytometry.dfci.harvard.edu

Building Layout

Contacts

Name Role Phone Email Location
Suzan Lazo
Director of Flow Cytometry
 
617-632-4571
 
suzan_lazo@dfci.harvard.edu
 
Mayer 584
 

Services

Name Description