Core - Longwood Medical Area CyTOF Mass Cytometer

Overview

  

Welcome to the Longwood Medical Area CyTOF Mass Cytometer page!  

 

The CyTOF is located on the 4th floor of the Jimmy Fund Building, Room 415 and 423.

 

CyTOF®2:  Time-of-Flight mass spectrometer for high-speed acquisition of highly multi-parametric single cell data.

 

Description

CyTOF®2 Mass Cytometer introduces the next generation of highly multi-parametric single cell analyzers which will afford new insights into the biology of complex cellular networks. It uses atomic mass spectrometry to allow simple, independent detection of up to 40 metal labeled antibodies based on currently available Conjugated Antibodies, MAXPAR® kits, and DNA intercalators.

 

CyTOF Signup and Access

Click here to signup for a DFCI iLab account if you do not already have one. Once you have a DFCI iLab account, request access to the CyTOF Core. 

 

Click here to subscribe to our mailing list!

     

   

Information for Users External to DFCI

 

Purchase Orders are to be made out to:

Dana Farber Cancer Institute

General Accounting, BP-430

450 Brookline Avenue

Boston, MA 02215-5450

 

Note to Partners, Harvard, Boston Children’s and Tufts researchers:

  • Partners Health Care (includes MGH, BWH, McLean, Spaulding, etc): choose vendor 0000008813
  • Harvard (includes HMS,Dental, Wyss, HSPH, etc.): choose vendor site: 01_PRIMARY
  • Tufts University: choose Supplier 0000000655
  • Boston Children’s Hospital: choose vendor 0000001886

 

 If you are using a PO from an institution other than one under which you signed in, you must stop and contact the DFCI iLab staff at sc_billing@dfci.harvard.edu

 

 

Equipment

  

Instrument Rates                            

 

DFCI & BWH Rates:                                                        $80.00/hour

Original Consortium Members (non-DFCI/BWH):           $120.00/hour

External Rates:                                                                $163.00/hour

Corporate Rates:                                                             $235.45/hour

 

Rates are calculated in 0.5 hour increments.

1 hour minimum reservation

Please see Policies

 

 

Project Consultation and Training Rates              

 

Applicable for New User Introduction, Protocol and Acquisition Consultation, and Instrument Training

 

DFCI & BWH Rates:                                                        $54.00/hour

Original Consortium Members (non-DFCI/BWH):           $80.00/hour

External Rates:                                                                $109.06/hour

Corporate Rates:                                                             $157.54/hour

 

Rates are calculated in 0.5 hour increments.

0.5 hour minimum reservation

Please see Policies

 

Bioinformatics Rates              

 

Applicable for Data Analysis, Training, Consultations, and Solution Development

 

DFCI & BWH Rates:                                                        $52.00/hour

Original Consortium Members (non-DFCI/BWH):           $78.00/hour

External Rates:                                                                $105.74/hour

Corporate Rates:                                                             $152.74/hour

 

Rates are calculated in 0.5 hour increments.

0.5 hour minimum reservation

Please see Policies

 

 

CyTOF Resources

 

Fluidigm Technical Literature  

 

Immunostaining protocols

Protocols for immunostaining with primary metal-tagged antibodies

 

Antibody Panel Kits 

Complete Surface and Intracellular kits from Fluidigm

 

Ir-intercalator Protocol

Ir-Intercalator is used to identify all cells in the experiemtn and is required in all samples.

Protocol for staining of intracellular components with an Ir-intercalator: 

 

Viability Marker Protocols 

Protocols for staining of intracellular components for viability.  

Rh103 Intercalator Protocols:   

Cisplatin Protocols 

 

Cell Cycle Protocols

 

MAXPAR® protocol
Protocol for preparing metal-labeled antibodies for cellular staining.

 

 

 

BWH CyTOF Antibody Core

 

Antibodies are available for purchase from The BWH CyTOF Antibody Core is located at BWH Thorn Building, Room 423.  

To access the complete antibody list, pricing and instructions on the request/ordering process, please contact Josh Keegan (jkeegan@partners.org) or Jay Dolan (jdolan6@partners.org) 

 

Laboratory Contact

 

Address:

Dana Farber CyTOF Core Facility

Attn:Nicole Paul

Dana-Farber Cancer Institute
450 Brookline Ave.
Jimmy Fund 415
Boston, MA 02215

 

Telephone & Email:

Email: CyTOF@dfci.harvard.edu

Phone: 617-632-6561

 

Building Layout

Contacts

Name Role Phone Email Location
Nicole Paul
CyTOF Specialist
 
617-632-6561
 
nicole_paul@dfci.harvard.edu
 
Jimmy Fund 415/423
 
Josh Keegan
BWH CyTOF Antibody Core
 
508-942-8291
 
JKeegan@partners.org
 
BWH Thorn 423
 
Hongye Liu
Data Scientist
 
617-632-6561
 
hongye_liu@dfci.harvard.edu
 
Smith #768
 

Available Equipment and Resources

CyTOF Instruments (2)
Data Analysis and Project Consultation (1)